Alizarin red is a laboratory dye used in analytical chemistry biological staining and research testing.
Alizarin Red S is a water-soluble anthraquinone dye and sodium salt of alizarin sulfonic acid, used extensively in histology, geology, and biochemistry for staining calcium deposits, cartilage, and bone. It identifies calcium via chelation, appearing bright red, and is commonly used in studies of osteogenic differentiation and mineralization.
Key Aspects of Alizarin Red S:
Applications:
Bone & Cartilage: Used to stain calcium deposits in tissue sections.
Cell Culture: Used for quantifying mineral deposits (calcification) in osteogenic cultures.
Geology: Used to differentiate carbonate minerals.
Chemical Properties: It is a reddish-brown powder with a solubility in water. It acts as a dye and, depending on pH, shows different colors (e.g., yellow to violet/red), providing a color change with chelation.
Mechanism: The sulphonate group allows for high solubility in water, distinguishing it from non-soluble alizarin.
Safety: It is Generally recognized as safe for laboratory use, but, as with any chemical, direct contact with eyes or skin should be avoided, and it should be kept away from drains.
Storage: Recommended to be stored at room temperature.
