Bromocresol green is a laboratory indicator dye used in analytical chemistry.
Bromocresol green is a triphenylmethane dye widely used as a pH indicator and a protein-binding stain in various chemical and biological applications.
Key Properties
Appearance: Beige to light brown or cream-colored powder.
Chemical Formula: C₂₁H₁₄Br₄O₅S.
Molar Mass: 698.01 g/mol.
Solubility: Sparingly soluble in water, but very soluble in ethanol, diethyl ether, and acetone.
pH Indication
Bromocresol green functions as a weak acid that changes color with changes in pH
Common Uses
Acid-Base Titrations: It is a reliable indicator for titrations, especially in the acidic to neutral range, due to its distinct color change.
Clinical Diagnostics: One of its most common uses is in clinical practice to measure serum albumin concentration in mammalian blood samples, which can indicate kidney or liver disease.
Electrophoresis: It is used as a tracking dye for DNA agarose gel electrophoresis.
Chromatography: In thin-layer chromatography (TLC), it is used to visualize acidic compounds like carboxylic acids, appearing as yellow spots on a blue background without the need for heating.
Microbiology: It is also incorporated into microbial growth media.
